I'm wondering if we can use Yakult as a yogurt starter to obtain drinkable yogurt?
Update: Yes, it is possible.
After a note in the comments, I tried researching and had to go no further than Wikipedia to see how it is done commercially: The incubation happens in the presence of glucose and continues for 7-8 days. I checked their source too, an article in an encyclopedia on food technology, and the information was correctly transcribed.
I decided to try it out. As I didn't have glucose, I poured in a random amount of agave syrup (a mixture of glucose and fructose). Indeed, at 8 hours (my standard incubation time for Lactobacillicus Bulgaricus yogurt) it was still completely liquid. But after 24 hours, it started looking good, and now, at hour 36, it has firmed up as normal yogurt.
The Shirota yogurt turned out to be quite interesting.
It looks like normal yogurt, with a small layer of whey on top, which looks somehow different from typical yogurt whey. The top slightly-fatty layer that builds on top of other yogurt is on top of the liquid layer and not below it. The colour is very slightly off-white, but nowhere near as orange as Yakult drinks.
The yogurt tastes differently from standard yogurt. It has a fruity smell, and is more sweet than sour, with bitter notes mixed in. I didn't notice acetic acid smells, the sourness must be predominantly lactic acid. The smell is rather subtle. The texture is unremarkable, just like standard Lactobacillicus yogurt.
At first, I was afraid that the long incubation time and the need for dextrose might mean that L. Shirota is difficult to grow, and might not work well under not-so-precisely controlled conditions, producing spoiled milk instead of yogurt. Now, having smelled the yogurt, I am pretty sure that there is a lot of the original Shirota culture growing there. My new hypothesis is that they need long growth times to achieve a very high concentration, so they can dilute it to make the drink and still get a strong taste.
I left the yogurt to incubate somewhat longer than a week. I was not happy with the result.
Some of the glasses got a band of light-reddish colour, reminiscent of commercial Yakult but slightly paler, while others got no such band. Those who had it had the band in the top third of the glass, but it was not the top layer.
The yogurt smelled quite sour. I tasted just a little bit, the taste was sour, but not as much as over fermented L. Bulgaricus yogurt. I didn't smell much acetic acid if any. There was a bit of sweetness in the taste, but no more than at the beginning, it felt. The Shirota-typical smell was subtle, not much stronger than at 36 hours.
After some more resarch, L. Shirota is sensitive to the absence of certain nutrients, mostly aminoacids and vitamins. See https://doi.org/10.1016/j.lwt.2019.108735 for details. But note that even without nutrients, they reached a good (for typical yogurt, not for yakult) concentration after 31 hours.
You can use most yogurts as a starter, no matter the brand. The only requirement is that they contain live culture, and all the ones marketed as a probiotic should have it.
It should give you normal yogurt, not a yogurt drink. You will have to follow a recipe for a yogurt-based drink if you want it liquid - just water and sugar to taste should do, of you want to mimic Yakult, or you can branch or into more interesting stuff.
1I tried yesterday. After 12 hours of incubation, it's not thick, which I don't mind because I want to make it drinkable. But it's not sour either. I think using Yakult doesn't work.– SeanApr 18, 2021 at 9:45
Lol, that is so „you“! Well done!– Stephie ♦Apr 24, 2021 at 12:24
The flavours will be a result of the metabolic processes of the particular strain used in the Yakult - much like beers have distinct flavours, the same applies to all fermentations. The length of fermentation will enhance these. I'm a little surprised at the length of ferment needed. I wonder what the phenotype is. If I can dig it out, i'll post it for those interested.– bob1May 6, 2021 at 10:09
@bob1 yes, I thought that different bacteria will produce different flavors. After my experiment, I also think that the Shirota strain is quite picky about its fermentation conditions - seeing how strong the specific flavor is in the diluted drink, they must have a much stronger culture going on in the commercial production, but I was not able to get that at home, while other strains are much more robust and make good yogurt under a wide range of fermentation conditions. If you have additional info you can post, I would be interested to read it.– rumtscho ♦May 6, 2021 at 10:15
@bob1 can you find out if it produces esters? The smell is very characteristic, but hard to describe beyond slightly sweet/fruity, and, well, estery. It feels like the main component is not an acid - certainly not lactic or acetic acid, these are present too, but there is something else that makes is different from other yogurts.– rumtscho ♦May 6, 2021 at 10:20