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I have been making kefir regularly for a couple years. Lately, however, my family's interest in it has waned and I have been using it less often and so feeding it less often.

I want to store some kefir grains so I don't lose them due to my own negligence. I've heard that grains can be frozen but I'm scared to kill some in the experiment.

What is a proven procedure for freezing (and subsequently rethawing) my kefir grains such that they stay viable?

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  • As a side-question, how do you prevent the kefir from developing little tiny, useless curds, and instead evenly thickening into something like yogurt? – BobMcGee Jul 6 '11 at 3:17
  • @BobMcGee, agitation and stirring should be enough to homogenise it if it is just a couple of days old. Otherwise, I would add more milk, ferment for half a day, strain off the curds to keep as a starter, and you should have a good smooth kefir. – user110084 Jun 8 '17 at 18:28
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Less complicated than the other method, but similar steps at the beginning. I have used two methods and both worked. I have heard success stories for grains in the freezer for over a year. No milk powder involved.

  • Wash the grains in both cases.

Methods:

  1. Put in fresh milk (the same you used before to make the kefir) and then freeze in a plastic container or so (I used a plastic bag)
  2. Let the grains dry. Not just with kitchen tissue, but actually leave it dry for an hour or two. Finally put it into kitchen tissue and wrap that in a plastic bag or so for freezing.

For thawing I left the grains in the fridge for half a day and then gave them directly into milk.

I tested both methods, as stated. Both worked just fine. In my case the max. time was more than 2 months. However, when "reactivating" the grains it took both some time and the results of the first two or three batches were not consumable (well, I guess they were, but just not pleasurable as kefir proper).

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    Thank you! I had read the instructions but was looking for personal experience. – Sobachatina Jul 9 '11 at 19:26
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One method for storing kefir grains for periods of up to 2 months, is by freezing spare grains.

To freeze effectively, wash the grains with pre-boiled COOLED water. Pat-dry the grains between pre-ironed cooled white toweling to remove excess moisture.

Place the grains in a jar or in a plastic bag, seal and put in the freezer. With milk grains, first add some dry milk powder, [DMP] adding enough to completely cover the grains with the DMP, seal jar or bag and then freeze.

DMP is mixed with the milk kefir-grains as a cryoprotectant agent to prevent freezer burn. Although I've found that kefir grains are viable for up to one year with this method, this length of time may completely remove the yeast component found in healthy kefir grains [if frozen for longer than 2 months, but not specifically]. Because of this potential, freezing kefir grains as explained above, is best performed for a period of no longer than 2 months.

If DMP is omitted for freezing milk kefir-grains, then a period of no longer than 1 month is recommended. Otherwise the yeast component of kefir grains may become damaged, especially if continual partial thawing and freezing due to poor freezer mechanism is involved.

To reactivate frozen kefir grains, thaw by placing the grains in a glass filled with cold water for a few minutes. Place the grains into a strainer and wash off any powdered milk that's adhered to the grains with cold water. http://users.sa.chariot.net.au/~dna/Makekefir.html#Storing_kefir-grains

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    Have you done this? I'm interested if anyone has proven this method. – Sobachatina Aug 26 '10 at 12:23
  • Sobachatina, reading Janelle's answer, in the 4th paragraph, it sounds like she's used this and found keffir grains were viable for 1 year frozen but with loss of yeast component. She recommends 2 months max for best results. – Jude Jun 8 '17 at 20:55
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I've successfully frozen and defrosted milk kefir grains. The defrosted kefir grains worked the first time culturing in fresh milk. I washed the kefir grains in filtered water till water was clear. Packed the washed kefir grains into zip lock snack bags with some plain filtered water and heat sealed the bag. I double bagged and heat sealed each bag. After 5 months I took the bag of frozen kefir grains and defrosted at room temperature overnight. In the morning I fed the kefir grains with some warm fresh milk and left to culture for 24 hours. They haven't looked back. Making beautiful kefir every day.

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  • Thank you for your experience! It's good to know that it works. – Sobachatina May 25 '12 at 15:50
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I have frozen dairy kefir grains in a 1/2 c canning jar with no rinsing, adding in a little kefir to surround grains. When I was ready to use, I left jar in refrigerator overnight and added to fresh milk in the morning. My grains have been frozen several times and reused in this manner. So far so good.

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To freeze milk kefir grains, I place the unwashed grains in a pint glass bottle and fill the jar 3/4 full with fresh milk, put on the lid and place in the freezer compartment.

To freeze water kefir grains, I do the same thing only I fill the jar 3/4 full with sugar water, (the same type as I use to make water kefir), put on the lid and place in the freezer. Leaving enouh room for the ice to expand safely in the jar.

When I want to reuse either of them, I take the jar out of the freezer and sit it on the refrigerator top until thoroughly thawed and then use as normal.

When I first reuse them, they always work immediately as if they had not been frozen at all.

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