Can food cooked by the sous vide method spoil once vacuum packed?
I sous vide spaghetti squash. I, then, vacuum pack the squash. Finally, I forget to put it in the freezer so it sits at room temperature for a day.
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Yes, it can both spoil and be unhealthy, even deadly. The micro organisms and toxins they can produce were there before you put it the bag and vacuum sealed it to cook it sous vide. If the temperature you cooked it at was not high enough to kill the micro organisms and destroy their toxins, they are still there. Just vacuum packing it a second time then leaving the package at room temperature has changed nothing. The food can still spoil and still make you sick or kill those who consume it. Leaving it at room temperature has allowed them to multiply/grow.