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I recently observed the effect of time of steeping on caffeine content in tea. We used High Pressure Liquid Chromatography to determine the levels of caffeine in Green Tea in samples that were … steeped for 1, 2, 4, 10, 15, 30, 60, and 120 mins. The concentration of caffeine over time did not show any significant trends. Our results suggest that caffeine content does not depend on the steeping time …
answered Oct 26 '13 by Bill Johnson